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PCR仪  凝胶成像系统  细胞计数仪   切胶仪   电子冰盒

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    Logos LUNA-FL™双荧光细胞计数仪

    LUNA-FL能够对原代细胞、外周血单核细胞、淋巴细胞、肿瘤细胞、神经元原代细胞、干细胞、酵母、脂肪和脂肪干细胞、牛奶中的体细胞、精子、藻类等难计数的细胞进行准确计数。

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    型号:

    LUNA-FL™

    LUNA-FL™

    品牌:

    Logos Biosystems

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    质保期:

    1年

    kg

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    • 技术参数
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      • 商品名称: Logos LUNA-FL™双荧光细胞计数仪
      • 商品编号: 95650
      • 品牌: Logos Biosystems
      • 型号: LUNA-FL™
      • 质保期: 1年
      • 应用: 生信分析
      • 类型: 普通凝胶成像系统
      • 国别: 韩国

      LUNA-FL能够对原代细胞、外周血单核细胞、淋巴细胞、肿瘤细胞、神经元原代细胞、干细胞、酵母、脂肪和脂肪干细胞、牛奶中的体细胞、精子、藻类等难计数的细胞进行准确计数。

         产品简介

      LUNA-FL是一款配备明场和双荧光通道的细胞计数仪,具有四种计数模式:明场细胞计数、荧光细胞计数、酵母细胞计数、转染效率计算,配备明场和双荧光光学元件,功能强大,可以评估细胞计数、存活率和GFP转染效率,可有效实现小于5um的细胞计数,可对除细菌以外的大多数细胞类型进行计数。能够对原代细胞、外周血单核细胞、淋巴细胞、肿瘤细胞、神经元原代细胞、干细胞、酵母、脂肪和脂肪干细胞、牛奶中的体细胞、精子、藻类等难计数的细胞进行准确计数。利用荧光染色技术大大提高了计数的准确性和稳定性,而且不受细胞类型或大小的限制。同时又将计数成本降到了最低。

         操作界面展示

          四种计数模式  ↑   

          自定义设置保存参数  ↑  

           具有稀释计算功能   ↑   

           计数结果显示   ↑   

         产品特点

           两个计数器合二为一     
      可以明场计数,也可以荧光模式进行计数

           与绝大多数细胞兼容      
      先进的成像软件和GFP以及RFP荧光光学系统,可非常灵敏地检测到荧光染色的细胞

           GFP 转染分析     

           计数时间快     
      荧光计数30秒,明场计数10秒

           非一次性计数板,可反复利用     
      也可选一次性计数板(T-Bond专利技术)

         验证过的细胞

         产品订购信息

      货号 产品描述 数量
      L20001 LUNA-FL™ Automated Fluorescence Cell Counter 1 套
      L12005 PhotonSlide™, 50 Slides   荧光计数板 / 50片 1 盒
      L12006 PhotonSlide™, 500 Slides  荧光计数板 / 500片 10 盒
      L12007 PhotonSlide™, 1000 Slides 荧光计数板 / 1000片 20 盒
      L12001 LUNA™ Cell Counting Slides, 50 Slides 1 盒
      L12002 LUNA™ Cell Counting Slides, 500 Slides 10 盒
      L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 盒
      L12011 LUNA™ Reusable Slide     可重复计数板 1 个
      L12012 LUNA™ Reusable Slides 2 个
      L12014 LUNA™ Reusable Slide Coverslips     盖玻片 10 个
      T13001 Trypan Blue Stain, 0.4%    台盼蓝染料 2 X 1 mL
      L13002 Erythrosin B Stain 2 x 1 mL
      F23001 Acridine Orange/Propidium Iodide Stain   预混荧光染料 2 x 0.5 mL
      F23002 Acridine Orange Stain 2 x 0.5 mL
      F23003 Propidium Iodide Stain 2 x 0.5 mL
      F23202 Yeast Viability Kit 1 1 kit
      - Propidium Iodide Stain for Yeast (1 x 0.5 mL)
      - Fluorescein Diacetate Stain (1 x 0.5 mL)
      - Cell Dilution Buffer (1 x 20 mL)
      - Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
      F23212 Cell Dilution Buffer 5 x 20 mL
      F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
      B13101 LUNA™ Standard Beads 2 x 1 mL
      F24003 LUNA™ IQ/OQ Protocol 1 copy
      P10001 LUNA™ Printer 1 unit
      P12001 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls

         产品发表文献

      ·  Brain Cancer Cell-derived Exosomes ProtectScopolamine-Induced Death of SH-SY5Y Neuron Cells.2021. Lee M. International Journal of HealthSciences and Research 10.36838/v3i1.8.

      ·  The Type and Source of Reactive Oxygen SpeciesInfluences the Outcome of Oxidative Stress in Cultured Cells.2021. Goffart S, Tikkanen P, Michell C,Wilson T, Pohjoismäki JLOLO.Cells 10(5):1075.

      ·  Immune Memory in Mild COVID-19 Patients andUnexposed Donors Reveals Persistent T Cell Responses After SARS-CoV-2Infection.2021. Ansari A, Arya R, Sachan S, Jha SN,Kalia A, Lall A, Sette A, Grifoni A, Weiskopf D, Coshic P, Sharma A, Gupta N.Frontiers in Immunology 12:636768.

      ·  The Small GTPase Arf6 Functions as a MembraneTether in a Chemically-Defined Reconstitution System.2021. Fujibayashi K, Mima J. Frontiers inCell and Developmental Biology 9:628910.

      ·  Flow cytometric quantification of apoptotic andproliferating cells applying an improved method for dissociation of spheroids.2021. Metzger W, Rösch B, Sossong D, Bubel M, Pohlemann T. CellBiology International 10.1002/cbin.11618. 

      ·  Paraphysoderma sedebokerense Infection in ThreeEconomically Valuable Microalgae: Host Preference Correlates with ParasiteFitness.2021. Alors D, Boussiba S, Zarka A. Journalof Fungi 7(2):100.

      ·  Role of TLR4 in persistent Leptospira interrogansinfection: a comparative in vivo study in mice.2021. Nair N, Guedes MS, Hajjar AM, Werts C,Gomes-Solecki M. Frontiers in Immunology 11:572999.

      ·  Protective effects of luteolin on the venousendothelium.2021. Assunção HCR, Cruz YMC, Bertolino JS, Garcia RCT,Fernandes L. Molecular and Cellular Biochemistry 476(4):1849–1859.

      ·  The Small GTPase Arf6 Functions as a MembraneTether in a Chemically-Defined Reconstitution System.2021. Fujibayashi K, Mima J. Frontiers inCell and Developmental Biology 9:628910.

      ·  Exposure-related, global alterations in innate andadaptive immunity; a consideration for re-use of non-human primates inresearch.2021. Bates FA, Duncan EH, Simmons M,Robinson T, Samineni S, Strbo N, Villasante E, Bergmann-Leitner E, WijayalathW. PeerJ 9:e10955.

      ·  Selective Proliferation of Highly FunctionalAdipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.2021. Mashiko T, Kanayama K, Saito N, ShiradoT, Asahi R, Mori M, Yoshimura K. Cells 10(3):560

      ·  High-throughput analysis of lung immune cells in acombined murine model of agriculture dust-triggered airway inflammation withrheumatoid arthritis.2021. Gaurav R, Mikuls TR, Thiele GM, NelsonAJ, Niu M, Guda C, Eudy JD, Barry AE, Wyatt TA, Romberger DJ, Duryee MJ,England BR, Poole JA. PLoS One 16(2):e0240707.

      ·  Inhibiting calpain 1 and 2 in cyclin G associatedkinase–knockout mice mitigates podocyte injury.2020. Tian X, Inoue K, Zhang Y, Wang Y,Sperati CJ, Pedigo CE, Zhao T, Yan M, Groener M, Moledina DG, Ebenezer K, Li W,Zhang Z, Liebermann DA, Greene L, Greer P, Parikh CR, Ishibe S. JCI Insight5(22):e142740.

      ·  Multi-Omics Resolves a Sharp Disease-State Shiftbetween Mild and Moderate COVID-19.2020. Su Y, Chen D, Yuan D, Lausted C, ChoiJ, Dai CL, Voillet V, Duvvuri VR, Scherler K, Troisch P, Baloni P, Qin G, SmithB, Kornilov SA, Rostomily C, Xu A, Li J, Dong S, Rothchild A, Zhou J, Murray K,Edmark R, Hong S, Heath JE, Earls J, Zhang R, Xie J, Li S, Roper R, Jones L,Zhou Y, Rowen L, Liu R, Mackay S, O'Mahony DS, Dale CR, Wallick JA, Algren HA,Zager MA; ISB-Swedish COVID19 Biobanking Unit, Wei W, Price ND, Huang S,Subramanian N, Wang K, Magis AT, Hadlock JJ, Hood L, Aderem A, Bluestone JA,Lanier LL, Greenberg PD, Gottardo R, Davis MM, Goldman JD, Heath JR. Cell183(6):1479-1495, e20.

      ·  Baculovirus-vectored precision delivery of largeDNA cargoes in human genomes.2020. Aulicino F, Pelosse M, Toelzer C, CapinJ, Meysami P, Dillingham MS, Schaffitzel C, Berger I. Biorxiv2020.08.17.253898.

      ·  Quantitative Assays Reveal Cell Fusion at MinimalLevels of SARS-CoV-2 Spike Protein and Fusion-from-Without.2020. Theuerkauf SA, Michels A, Riechert V,Maier TJ, Flory E, Cichutek K, Buchholz CJ. Biorxiv 2020.10.15.340604.

      ·  Identification of genomic enhancers through spatialintegration of single‐cell transcriptomics and epigenomics.2020. Bravo González-Blas C, Quan XJ,Duran-Romaña R,Taskiran II, Koldere D, Davie K, Christiaens V, Makhzami S, Hulselmans G, deWaegeneer M, Mauduit D, Poovathingal S, Aibar S, Aerts S. Molecular SystemsBiology 16(5):e9438.

      ·  Safety and Tolerability of Stromal VascularFraction Combined with β-Tricalcium Phosphate in Posterior Lumbar InterbodyFusion: Phase I Clinical Trial.2020. Choi UY, Kim KT, Kim KG, Lim SH, KimYJ, Sohn S, Sheen SH, Heo CY, Han I. Cells 9(10):2250.

      ·  Real-Time Quantitative Reverse Transcription PCRfor Detection of Opioid Receptors in Immune Cells.2020. Celik MÖ, Labuz D, Machelska H. Methods in molecularbiology (Clifton, N.J.) 2201:83–95.

      ·  Selection of microRNAs in extracellular vesiclesfor diagnosis of malignant pleural mesothelioma by in vitro analysis.2020. Jotatsu T, Izumi H, Morimoto Y, YateraK. Oncology Reports 44(5):2198–2210. 

      ·  Establishment of an In Vitro Model of PersistentChicken Anemia Virus Infection.2020. Van Dong H, Tran GTH, Trinh DQ, TakedaY, Ogawa H, Imai K. Pathogens (Basel, Switzerland) 9(10):842.

      ·  High‐Throughput Magnetic Actuation Platform forEvaluating the Effect of Mechanical Force on 3D Tumor Microenvironment.2020. Enríquez A, Libring S, Field TC,Jimenez J, Lee T, Park H, Satoski D, Wendt MK, Calve S, Tepole AB, Solorio L,Lee H. Advanced Functional Materials 10.1002/adfm.202005021. 

      ·  Safety and Tolerability of Stromal VascularFraction Combined with β-Tricalcium Phosphate in Posterior Lumbar InterbodyFusion: Phase I Clinical Trial.2020. Choi UY, Kim KT, Kim KG, Lim SH, KimYJ, Sohn S, Sheen SH, Heo CY, Han I. Cells 9(10):2250.

      ·  Chemically modified mRNA nucleofection of primaryhuman T cells.2020. Thuille N, Sajinovic T, Siegmund K,Baier G. Journal of Immunological Methods 112878.

      ·  Caloric Restriction Reprograms the Single-CellTranscriptional Landscape of Rattus Norvegicus Aging.2020. Ma S, Sun S, Geng L, Song M, Wang W, YeY, Ji Q, Zou Z, Wang S, He X, Li W, Esteban CR, Long X, Guo G, Chan P, Zhou Q,Belmonte JCI, Zhang W, Qu J, Liu GH. Cell 180(5):984–1001.e22.

      ·  Effects of Gelatin Methacrylate Bio-inkConcentration on Mechano-Physical Properties and Human Dermal FibroblastBehavior.2020. Shie MY, Lee JJ, Ho CC, Yen SY, Ng HY,Chen YW. Polymers (Basel) 12(9):E1930. 

      ·  Single-cell transcriptomic atlas of primatecardiopulmonary aging.2020. Ma S, Sun S, Li J, Fan Y, Qu J, Sun L,Wang S, Zhang Y, Yang S, Liu Z, Wu Z, Zhang S, Wang Q, Zheng A, Duo S, Yu Y,Belmonte JCI, Chan P, Zhou Q, Song M, Zhang W, Liu GH. Cell Research10.1038/s41422-020-00412-6.

      ·  Quantitative Detection of Plasmodium falciparumUsing, LUNA-FL, A Fluorescent Cell Counter.2020. Hashimoto M, Yokota K, Kajimoto K,Matsumoto M, Tatsumi A, Yamamoto K, Hyodo T, Matsushita K, Minakawa N, Mita T,Oka H, Kataoka M. Microorganisms 4;8(9):E1356.

      ·  Post-copulatory genetic matchmaking: HLA-dependenteffects of cervical mucus on human sperm function.2020. Jokiniemi A, Magris M, Ritari J, KuusipaloL, Lundgren T, Partanen J, Kekäläinen J. Biological Sciences287(1933):20201682.

      ·  Human Mesenchymal Stem Cell Hydrogen SulfideProduction Critically Impacts the Release of Other Paracrine Mediators AfterInjury.2020. Markel TA, Drucker NA, Jensen AR, OlsonKR. The Journal of Surgical Research 254:75-82. 

      ·  Analytical comparison of structural changes ofplastic cell-based therapeutic drug storage containers when exposed tocryogenic environments.2020. Park JY, Lee DM, Lee J, Lee SR, YoonGS. Journal of the Korea Society of Die & Mold Engineering 14(2):1-6.

      ·  High-Content Immunophenotyping and HierarchicalClustering Reveal Sources of Heterogeneity and New Surface Markers of HumanBlood Monocyte Subsets.2020. Hoffmann J, Fišer K, Liebetrau C, Staubach N, Kost D, VossS, Heiden AZ, Dörr O,Lipps C, Nef HM, Möllmann H,Hamm CW, Keller T, Troidl C. Thrombosis and Haemostasis 120(1):141-155.

      ·  Single-cell transcriptome analysis of the Akimbamouse retina reveals cell-type-specific insights into the pathobiology of diabeticretinopathy.2020. Van Hove I, De Groef L, Boeckx B,Modave E, Hu TT, Beets K, Etienne I, Van Bergen T, Lambrechts D, Moons L, FeyenJHM, Porcu M. Diabetologia doi: 10.1007/s00125-020-05218-0.

      ·  Similarities and Differences in the Effects ofToxic Concentrations of Cadmium and Chromium on the Structure and Functions ofThylakoid Membranes in Chlorella variabilis.2020. Zsiros O, Nagy G, Patai R, Solymosi K,Gasser U, Polgár TF, Garab G, Kovács L, Hörcsik ZT. Frontiers in Plant Science 11:1006.

      ·  Vascular occlusion by neutrophil extracellulartraps in COVID-19.2020. Leppkes M, Knopf J, Naschberger E,Lindemann A, Singh J, Herrmann I, Stürzl M, Staats L, Mahajan A, Schauer C,Kremer AN, Völkl S,Amann K, Evert K, Falkeis C, Wehrfritz A, Rieker RJ, Hartmann A, Kremer AE,Neurath MF, Herrmann M. EBioMedicine 58:102925.

      ·  Optimal Initial Cell Density that Yields theHighest Number of Primary Synovial Mesenchymal Stem Cells in a ClinicalSetting.2020. Horiuchi K, Mizuno M, Katano H, Endo K,Ozeki N, Tsuji K, Koga H, Sekiya I. Stem Cell & Developmental Cell Biologydoi: 10.21203/rs.3.rs-28311/v1. 

      ·  Photosynthetic characterization of twoNannochloropsis species and its relevance to outdoor cultivation.2020. Vonshak A, Novoplansky N, Benavides AM,Torzillo G, Beardall J, Palacios YM. Journal of Applied Phycologyhttps://doi.org/10.1007/s10811-019-01985-5.

      ·  Increased O-GlcNAcylation of c-Myc Promotes Pre-BCell Proliferation.2020. Lee DH, Kwon NE, Lee WJ, Lee MS, KimDJ, Kim JH, Park SK. Cells 9(1):158.

      ·  Inhibition of the PI3K-AKT-mTOR pathway suppressesthe adipocyte-mediated proliferation and migration of breast cancer cells.2020. Park JY, Kang SE, Ahn KS, Um JY, YangWM, Yun M, Lee SG. Journal of Cancer 2020;11(9):2552‐2559.

      ·  Effects of butyrate supplementation on bloodglucagon-like peptide-2 concentration and gastrointestinal functions oflactating dairy cows fed diets differing in starch content.2020. Fukumori R, Oba M, Izumi K, Otsuka M,Suzuki K, Gondaira S, Higuchi H, Oikawa S. Journal of Dairy Science 103(4):3656‐3667.

      ·  Curvature-sensitive trans-assembly of humanAtg8-family proteins in autophagy-related membrane tethering.2020. Taniguchi S, Toyoshima M, Takamatsu T,Mima J. Protein Science : A Publication of the Protein Society10.1002/pro.3828. 

      ·  A Comparison of Ovine Facial and Limb Muscle as aPrimary Cell Source for Engineered Skeletal Muscle.2020. Rodriguez BL, Nguyen MH, Armstrong RE,Vega-Soto EE, Polkowski PM, Larkin LM. Tissue Engineering Part A26(3-4):167‐177.

      ·  Uptake and excretion dynamics of gold nanoparticlesin cancer cells and fibroblasts.2020. Ivošev V, Sánchez GJ, Stefancikova L,Haidar DA, González Vargas CR, Yang X, Bazzi R, Porcel E,Roux S, Lacombe S. Nanotechnology 31(13):135102.

       

    •    技术参数

      相机像素: 500万像素
      激发波长: 470±20nm
      发射波长: 525±25nm,600nm(LP)
      光源 蓝光LED/绿光LED
      细胞计数浓度范围: 5x104 ~ 1x107个/ml
      样品体积 10ul
      计数时间 30s
      样品大小范围: 细胞直径:1~90μm;最佳计数范围:5-60um
      LCD显示屏 7英寸
      体积(WxDxH) 22x21x9cm
      重量 1.8Kg

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